ID   NS3B_CVHSA              Reviewed;         154 AA.
AC   P59633; Q7TFA9; Q80BV4;
DT   23-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT   23-APR-2003, sequence version 1.
DT   22-APR-2020, entry version 74.
DE   RecName: Full=Non-structural protein 3b;
DE            Short=ns3b;
DE   AltName: Full=Accessory protein 3b;
DE   AltName: Full=Protein X2;
GN   ORFNames=3b;
OS   Human SARS coronavirus (SARS-CoV) (Severe acute respiratory syndrome
OS   coronavirus).
OC   Viruses; Riboviria; Nidovirales; Cornidovirineae; Coronaviridae;
OC   Orthocoronavirinae; Betacoronavirus; Sarbecovirus.
OX   NCBI_TaxID=694009;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
OH   NCBI_TaxID=9675; Paguma larvata (Masked palm civet).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate Urbani;
RX   PubMed=12730500; DOI=10.1126/science.1085952;
RA   Rota P.A., Oberste M.S., Monroe S.S., Nix W.A., Campagnoli R.,
RA   Icenogle J.P., Penaranda S., Bankamp B., Maher K., Chen M.-H., Tong S.,
RA   Tamin A., Lowe L., Frace M., DeRisi J.L., Chen Q., Wang D., Erdman D.D.,
RA   Peret T.C.T., Burns C., Ksiazek T.G., Rollin P.E., Sanchez A., Liffick S.,
RA   Holloway B., Limor J., McCaustland K., Olsen-Rasmussen M., Fouchier R.,
RA   Guenther S., Osterhaus A.D.M.E., Drosten C., Pallansch M.A., Anderson L.J.,
RA   Bellini W.J.;
RT   "Characterization of a novel coronavirus associated with severe acute
RT   respiratory syndrome.";
RL   Science 300:1394-1399(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate Tor2;
RX   PubMed=12730501; DOI=10.1126/science.1085953;
RA   Marra M.A., Jones S.J.M., Astell C.R., Holt R.A., Brooks-Wilson A.,
RA   Butterfield Y.S.N., Khattra J., Asano J.K., Barber S.A., Chan S.Y.,
RA   Cloutier A., Coughlin S.M., Freeman D., Girn N., Griffith O.L., Leach S.R.,
RA   Mayo M., McDonald H., Montgomery S.B., Pandoh P.K., Petrescu A.S.,
RA   Robertson A.G., Schein J.E., Siddiqui A., Smailus D.E., Stott J.M.,
RA   Yang G.S., Plummer F., Andonov A., Artsob H., Bastien N., Bernard K.,
RA   Booth T.F., Bowness D., Czub M., Drebot M., Fernando L., Flick R.,
RA   Garbutt M., Gray M., Grolla A., Jones S., Feldmann H., Meyers A.,
RA   Kabani A., Li Y., Normand S., Stroher U., Tipples G.A., Tyler S.,
RA   Vogrig R., Ward D., Watson B., Brunham R.C., Krajden M., Petric M.,
RA   Skowronski D.M., Upton C., Roper R.L.;
RT   "The genome sequence of the SARS-associated coronavirus.";
RL   Science 300:1399-1404(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate CUHK-Su10, and Isolate CUHK-W1;
RX   PubMed=12853594; DOI=10.1056/nejm200307103490216;
RA   Tsui S.K.W., Chim S.S.C., Lo Y.M.D.;
RT   "Coronavirus genomic-sequence variations and the epidemiology of the severe
RT   acute respiratory syndrome.";
RL   N. Engl. J. Med. 349:187-188(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate HKU-39849;
RX   PubMed=12876307; DOI=10.1177/15353702-0322807-13;
RA   Zeng F.Y., Chan C.W., Chan M.N., Chen J.D., Chow K.Y.C., Hon C.C.C.,
RA   Hui R.K.H., Li J., Li V.Y.Y., Wang C.Y., Wang P.Y., Guan Y., Zheng B.,
RA   Poon L.L.M., Chan K.H., Yuen K.Y., Peiris J.S.M., Leung F.C.;
RT   "The complete genome sequence of severe acute respiratory syndrome
RT   coronavirus strain HKU-39849 (HK-39).";
RL   Exp. Biol. Med. 228:866-873(2003).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate BJ01, Isolate BJ02, Isolate BJ03, Isolate BJ04, and
RC   Isolate GD01;
RA   Qin E., Zhu Q., Yu M., Fan B., Chang G., Si B., Yang B., Peng W., Jiang T.,
RA   Liu B., Deng Y., Liu H., Zhang Y., Wang C., Li Y., Gan Y., Li X., Lu F.,
RA   Tan G., Yang R., Cao W.S., Wang J., Chen W., Cong L., Deng Y., Dong W.,
RA   Han Y., Hu W., Lei M., Li C., Li G., Li G., Li H., Li S., Li S., Li W.,
RA   Li W., Lin W., Liu J., Liu Z., Lu H., Ni P., Qi Q., Sun Y., Tang L.,
RA   Tong Z., Wang J., Wang X., Wu Q., Xi Y., Xu Z., Yang L., Ye C., Ye J.,
RA   Zhang B., Zhang F., Zhang J., Zhang X., Zhou J., Yang H.;
RL   Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate TW1;
RA   Yeh S.-H., Kao C.-L., Tsai C.-Y., Liu C.-J., Chen D.-S., Chen P.-J.;
RT   "The complete genome of SARS coronavirus clone TW1.";
RL   Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN   [7]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate FRA;
RA   Eickmann M., Becker S., Klenk H.-D., Doerr H.W., Stadler K., Censini S.,
RA   Guidotti S., Masignani V., Scarselli M., Mora M., Donati C., Han J.,
RA   Song H.C., Abrignani S., Covacci A., Rappuoli R.;
RT   "SARS virus is a close relative of type II coronaviruses.";
RL   Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN   [8]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate Frankfurt 1;
RA   Thiel V., Hertzig T., Putics A., Ivanov K.A., Schelle B., Bayer S.,
RA   Scheiner B., Weinand H., Weissbrich B., Ziebuhr J.;
RL   Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN   [9]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate Shanghai QXC1;
RA   Yuan Z., Zhang X., Hu Y., Lan S., Wang H., Zhou Z., Wen Y.;
RT   "Analysis of SARS coronavirus genome in Shanghai isolates.";
RL   Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN   [10]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate Taiwan TC1, Isolate Taiwan TC2, and Isolate Taiwan TC3;
RA   Chang J.-G.C., Lin T.-H., Chen C.-M., Lin C.-S., Chan W.-L., Shih M.-C.;
RL   Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
RN   [11]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate TWH, Isolate TWJ, Isolate TWK, Isolate TWS, and Isolate TWY;
RA   Shu H.Y., Wu K.M., Tsai S.F.;
RT   "The complete genome of SARS coronavirus TWH.";
RL   Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
RN   [12]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate HSR 1;
RA   Canducci F., Clementi M., Poli G., Vicenzi E.;
RL   Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
RN   [13]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate AS;
RA   Balotta C., Corvasce S., Violin M., Galli M., Moroni M., Vigevani G.M.,
RA   Ruan Y.J., Salemi M.;
RL   Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
RN   [14]
RP   FUNCTION.
RX   PubMed=16107218; DOI=10.1186/1743-422x-2-66;
RA   Yuan X., Shan Y., Zhao Z., Chen J., Cong Y.;
RT   "G0/G1 arrest and apoptosis induced by SARS-CoV 3b protein in transfected
RT   cells.";
RL   Virol. J. 2:66-66(2005).
RN   [15]
RP   SUBCELLULAR LOCATION.
RX   PubMed=16046244; DOI=10.1016/j.virusres.2005.06.001;
RA   Yuan X., Yao Z., Shan Y., Chen B., Yang Z., Wu J., Zhao Z., Chen J.,
RA   Cong Y.;
RT   "Nucleolar localization of non-structural protein 3b, a protein
RT   specifically encoded by the severe acute respiratory syndrome
RT   coronavirus.";
RL   Virus Res. 114:70-79(2005).
RN   [16]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=16682811;
RA   Yuan X., Shan Y., Yao Z., Li J., Zhao Z., Chen J., Cong Y.;
RT   "Mitochondrial location of severe acute respiratory syndrome coronavirus 3b
RT   protein.";
RL   Mol. Cells 21:186-191(2006).
RN   [17]
RP   INTERACTION WITH HOST RUNX1.
RX   PubMed=22253733; DOI=10.1371/journal.pone.0029542;
RA   Varshney B., Agnihothram S., Agnihotram S., Tan Y.J., Baric R., Lal S.K.;
RT   "SARS coronavirus 3b accessory protein modulates transcriptional activity
RT   of RUNX1b.";
RL   PLoS ONE 7:E29542-E29542(2012).
CC   -!- FUNCTION: Induces host cell G0/G1 arrest and apoptosis.
CC       {ECO:0000269|PubMed:16107218, ECO:0000269|PubMed:16682811}.
CC   -!- SUBUNIT: Interacts with host RUNX1 isoform b.
CC       {ECO:0000269|PubMed:22253733}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus, host nucleolus
CC       {ECO:0000269|PubMed:16046244}. Host mitochondrion
CC       {ECO:0000269|PubMed:16682811}.
DR   EMBL; AY278741; AAP13447.1; -; Genomic_RNA.
DR   EMBL; AY274119; AAP41039.1; -; Genomic_RNA.
DR   EMBL; AY282752; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   EMBL; AY278554; AAP13569.1; -; Genomic_RNA.
DR   EMBL; AY278491; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   EMBL; AY278487; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   EMBL; AY278488; AAP30032.1; -; Genomic_RNA.
DR   EMBL; AY278489; AAP51229.1; -; Genomic_RNA.
DR   EMBL; AY278490; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   EMBL; AY279354; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   EMBL; AY291451; AAP37019.1; -; Genomic_RNA.
DR   EMBL; AY310120; AAP50487.1; -; Genomic_RNA.
DR   EMBL; AY291315; AAP33699.1; -; Genomic_RNA.
DR   EMBL; AY463059; AAR86789.1; -; Genomic_RNA.
DR   EMBL; AY338174; AAQ01599.1; -; Genomic_RNA.
DR   EMBL; AY338175; AAQ01611.1; -; Genomic_RNA.
DR   EMBL; AY348314; AAP97884.1; -; Genomic_RNA.
DR   EMBL; AP006557; BAC81350.1; -; Genomic_RNA.
DR   EMBL; AP006558; BAC81364.1; -; Genomic_RNA.
DR   EMBL; AP006559; BAC81378.1; -; Genomic_RNA.
DR   EMBL; AP006560; BAC81392.1; -; Genomic_RNA.
DR   EMBL; AP006561; BAC81406.1; -; Genomic_RNA.
DR   EMBL; AY323977; AAP72976.1; -; Genomic_RNA.
DR   EMBL; AY427439; AAQ94062.1; -; Genomic_RNA.
DR   RefSeq; NP_828853.1; NC_004718.3.
DR   SMR; P59633; -.
DR   IntAct; P59633; 4.
DR   PRIDE; P59633; -.
DR   GeneID; 1489670; -.
DR   KEGG; vg:1489670; -.
DR   Proteomes; UP000000354; Genome.
DR   Proteomes; UP000103670; Genome.
DR   Proteomes; UP000109640; Genome.
DR   Proteomes; UP000116947; Genome.
DR   Proteomes; UP000121636; Genome.
DR   Proteomes; UP000131569; Genome.
DR   Proteomes; UP000131955; Genome.
DR   Proteomes; UP000137377; Genome.
DR   Proteomes; UP000138690; Genome.
DR   Proteomes; UP000143093; Genome.
DR   Proteomes; UP000145651; Genome.
DR   Proteomes; UP000146108; Genome.
DR   Proteomes; UP000146181; Genome.
DR   Proteomes; UP000146296; Genome.
DR   Proteomes; UP000148194; Genome.
DR   Proteomes; UP000153467; Genome.
DR   Proteomes; UP000160648; Genome.
DR   Proteomes; UP000164441; Genome.
DR   Proteomes; UP000172416; Genome.
DR   GO; GO:0033650; C:host cell mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0044196; C:host cell nucleolus; IEA:UniProtKB-SubCell.
DR   GO; GO:0039526; P:modulation by virus of host apoptotic process; IEA:UniProtKB-KW.
DR   GO; GO:0039646; P:modulation by virus of host G0/G1 transition checkpoint; IEA:UniProtKB-KW.
DR   GO; GO:0001817; P:regulation of cytokine production; IEP:CACAO.
DR   InterPro; IPR022117; SARS_3b.
DR   Pfam; PF12383; SARS_3b; 1.
PE   1: Evidence at protein level;
KW   Apoptosis; G0/G1 host cell cycle checkpoint dysregulation by virus;
KW   Host mitochondrion; Host nucleus; Host-virus interaction;
KW   Modulation of host cell apoptosis by virus;
KW   Modulation of host cell cycle by virus; Reference proteome.
FT   CHAIN           1..154
FT                   /note="Non-structural protein 3b"
FT                   /id="PRO_0000106132"
FT   REGION          80..138
FT                   /note="Mitochondrial targeting signal"
FT   REGION          134..154
FT                   /note="Nucleolar targeting"
FT   MOTIF           135..153
FT                   /note="Bipartite nuclear localization signal"
FT                   /evidence="ECO:0000255"
FT   VARIANT         31
FT                   /note="K -> Q (in strain: Isolate GD01)"
FT   VARIANT         99
FT                   /note="T -> I (in strain: Isolate BJ02)"
FT   VARIANT         115
FT                   /note="L -> Q (in strain: Isolate GD01)"
FT   VARIANT         121
FT                   /note="Q -> P (in strain: Isolate BJ01 and Isolate BJ04)"
SQ   SEQUENCE   154 AA;  17750 MW;  82AB0B68F11060A8 CRC64;
     MMPTTLFAGT HITMTTVYHI TVSQIQLSLL KVTAFQHQNS KKTTKLVVIL RIGTQVLKTM
     SLYMAISPKF TTSLSLHKLL QTLVLKMLHS SSLTSLLKTH RMCKYTQSTA LQELLIQQWI
     QFMMSRRRLL ACLCKHKKVS TNLCTHSFRK KQVR
//