ID   ORF9B_CVHSA             Reviewed;          98 AA.
AC   P59636; Q7T6R2;
DT   23-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT   23-APR-2003, sequence version 1.
DT   22-APR-2020, entry version 92.
DE   RecName: Full=Protein 9b;
DE   AltName: Full=Accessory protein 9b;
DE   AltName: Full=ORF-9b;
GN   ORFNames=9b;
OS   Human SARS coronavirus (SARS-CoV) (Severe acute respiratory syndrome
OS   coronavirus).
OC   Viruses; Riboviria; Nidovirales; Cornidovirineae; Coronaviridae;
OC   Orthocoronavirinae; Betacoronavirus; Sarbecovirus.
OX   NCBI_TaxID=694009;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
OH   NCBI_TaxID=9675; Paguma larvata (Masked palm civet).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate Urbani;
RX   PubMed=12730500; DOI=10.1126/science.1085952;
RA   Rota P.A., Oberste M.S., Monroe S.S., Nix W.A., Campagnoli R.,
RA   Icenogle J.P., Penaranda S., Bankamp B., Maher K., Chen M.-H., Tong S.,
RA   Tamin A., Lowe L., Frace M., DeRisi J.L., Chen Q., Wang D., Erdman D.D.,
RA   Peret T.C.T., Burns C., Ksiazek T.G., Rollin P.E., Sanchez A., Liffick S.,
RA   Holloway B., Limor J., McCaustland K., Olsen-Rasmussen M., Fouchier R.,
RA   Guenther S., Osterhaus A.D.M.E., Drosten C., Pallansch M.A., Anderson L.J.,
RA   Bellini W.J.;
RT   "Characterization of a novel coronavirus associated with severe acute
RT   respiratory syndrome.";
RL   Science 300:1394-1399(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate Tor2;
RX   PubMed=12730501; DOI=10.1126/science.1085953;
RA   Marra M.A., Jones S.J.M., Astell C.R., Holt R.A., Brooks-Wilson A.,
RA   Butterfield Y.S.N., Khattra J., Asano J.K., Barber S.A., Chan S.Y.,
RA   Cloutier A., Coughlin S.M., Freeman D., Girn N., Griffith O.L., Leach S.R.,
RA   Mayo M., McDonald H., Montgomery S.B., Pandoh P.K., Petrescu A.S.,
RA   Robertson A.G., Schein J.E., Siddiqui A., Smailus D.E., Stott J.M.,
RA   Yang G.S., Plummer F., Andonov A., Artsob H., Bastien N., Bernard K.,
RA   Booth T.F., Bowness D., Czub M., Drebot M., Fernando L., Flick R.,
RA   Garbutt M., Gray M., Grolla A., Jones S., Feldmann H., Meyers A.,
RA   Kabani A., Li Y., Normand S., Stroher U., Tipples G.A., Tyler S.,
RA   Vogrig R., Ward D., Watson B., Brunham R.C., Krajden M., Petric M.,
RA   Skowronski D.M., Upton C., Roper R.L.;
RT   "The genome sequence of the SARS-associated coronavirus.";
RL   Science 300:1399-1404(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate CUHK-Su10, and Isolate CUHK-W1;
RX   PubMed=12853594; DOI=10.1056/nejm200307103490216;
RA   Tsui S.K.W., Chim S.S.C., Lo Y.M.D.;
RT   "Coronavirus genomic-sequence variations and the epidemiology of the severe
RT   acute respiratory syndrome.";
RL   N. Engl. J. Med. 349:187-188(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate HKU-39849;
RX   PubMed=12876307; DOI=10.1177/15353702-0322807-13;
RA   Zeng F.Y., Chan C.W., Chan M.N., Chen J.D., Chow K.Y.C., Hon C.C.C.,
RA   Hui R.K.H., Li J., Li V.Y.Y., Wang C.Y., Wang P.Y., Guan Y., Zheng B.,
RA   Poon L.L.M., Chan K.H., Yuen K.Y., Peiris J.S.M., Leung F.C.;
RT   "The complete genome sequence of severe acute respiratory syndrome
RT   coronavirus strain HKU-39849 (HK-39).";
RL   Exp. Biol. Med. 228:866-873(2003).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate BJ01, Isolate BJ02, Isolate BJ03, Isolate BJ04, and
RC   Isolate GD01;
RA   Qin E., Zhu Q., Yu M., Fan B., Chang G., Si B., Yang B., Peng W., Jiang T.,
RA   Liu B., Deng Y., Liu H., Zhang Y., Wang C., Li Y., Gan Y., Li X., Lu F.,
RA   Tan G., Yang R., Cao W.S., Wang J., Chen W., Cong L., Deng Y., Dong W.,
RA   Han Y., Hu W., Lei M., Li C., Li G., Li G., Li H., Li S., Li S., Li W.,
RA   Li W., Lin W., Liu J., Liu Z., Lu H., Ni P., Qi Q., Sun Y., Tang L.,
RA   Tong Z., Wang J., Wang X., Wu Q., Xi Y., Xu Z., Yang L., Ye C., Ye J.,
RA   Zhang B., Zhang F., Zhang J., Zhang X., Zhou J., Yang H.;
RL   Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate TW1;
RA   Yeh S.-H., Kao C.-L., Tsai C.-Y., Liu C.-J., Chen D.-S., Chen P.-J.;
RT   "The complete genome of SARS coronavirus clone TW1.";
RL   Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN   [7]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate FRA;
RA   Eickmann M., Becker S., Klenk H.-D., Doerr H.W., Stadler K., Censini S.,
RA   Guidotti S., Masignani V., Scarselli M., Mora M., Donati C., Han J.,
RA   Song H.C., Abrignani S., Covacci A., Rappuoli R.;
RT   "SARS virus is a close relative of type II coronaviruses.";
RL   Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN   [8]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate Frankfurt 1;
RX   PubMed=12917450; DOI=10.1099/vir.0.19424-0;
RA   Thiel V., Ivanov K.A., Putics A., Hertzig T., Schelle B., Bayer S.,
RA   Weissbrich B., Snijder E.J., Rabenau H., Doerr H.W., Gorbalenya A.E.,
RA   Ziebuhr J.;
RT   "Mechanisms and enzymes involved in SARS coronavirus genome expression.";
RL   J. Gen. Virol. 84:2305-2315(2003).
RN   [9]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate ZJ01;
RX   PubMed=14527350;
RA   Li L., Wang Z., Lu Y., Bao Q., Chen S., Wu N., Cheng S., Weng J., Zhang Y.,
RA   Yan J., Mei L., Wang X., Zhu H., Yu Y., Zhang M., Li M., Yao J., Lu Q.,
RA   Yao P., Bo X., Wo J., Wang S., Hu S.;
RT   "Severe acute respiratory syndrome-associated coronavirus genotype and its
RT   characterization.";
RL   Chin. Med. J. 116:1288-1292(2003).
RN   [10]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate TWH, Isolate TWJ, Isolate TWK, Isolate TWS, and Isolate TWY;
RA   Shu H.Y., Wu K.M., Tsai S.F.;
RT   "The complete genome of SARS coronavirus TWH.";
RL   Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
RN   [11]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate HSR 1;
RA   Canducci F., Clementi M., Poli G., Vicenzi E.;
RL   Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
RN   [12]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate AS;
RA   Balotta C., Corvasce S., Violin M., Galli M., Moroni M., Vigevani G.M.,
RA   Ruan Y.J., Salemi M.;
RL   Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
RN   [13]
RP   X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 9-98, AND SUBCELLULAR LOCATION.
RX   PubMed=16843897; DOI=10.1016/j.str.2006.05.012;
RA   Meier C., Aricescu A.R., Assenberg R., Aplin R.T., Gilbert R.J.C.,
RA   Grimes J.M., Stuart D.I.;
RT   "The crystal structure of ORF-9b, a lipid binding protein from the SARS
RT   coronavirus.";
RL   Structure 14:1157-1165(2006).
CC   -!- SUBUNIT: Homodimer.
CC   -!- INTERACTION:
CC       P59636; P59636: 9b; NbExp=3; IntAct=EBI-9021274, EBI-9021274;
CC       P59636; Q7TLC7: ORF14; NbExp=3; IntAct=EBI-9021274, EBI-25488942;
CC       P59636; PRO_0000037320 [P0C6X7]: rep; NbExp=3; IntAct=EBI-9021274, EBI-25487328;
CC   -!- SUBCELLULAR LOCATION: Host cytoplasmic vesicle membrane
CC       {ECO:0000269|PubMed:16843897}; Peripheral membrane protein
CC       {ECO:0000269|PubMed:16843897}. Host cytoplasm
CC       {ECO:0000269|PubMed:16843897}. Note=Binds non-covalently to
CC       intracellular lipid bilayers.
CC   -!- MISCELLANEOUS: The gene encoding this protein is included within the N
CC       gene (alternative ORF).
CC   -!- SIMILARITY: Belongs to the coronavirus group 2 protein 9b family.
CC       {ECO:0000305}.
DR   EMBL; AY278741; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   EMBL; AY274119; AAP41048.1; -; Genomic_RNA.
DR   EMBL; AY282752; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   EMBL; AY278554; AAP13574.1; -; Genomic_RNA.
DR   EMBL; AY278491; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   EMBL; AY278487; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   EMBL; AY278488; AAP30038.1; -; Genomic_RNA.
DR   EMBL; AY278489; AAP51235.1; -; Genomic_RNA.
DR   EMBL; AY278490; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   EMBL; AY279354; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   EMBL; AY291451; AAP37025.1; -; Genomic_RNA.
DR   EMBL; AY310120; AAP50496.1; -; Genomic_RNA.
DR   EMBL; AY291315; AAP33708.1; -; Genomic_RNA.
DR   EMBL; AY290752; AAP69659.1; -; Genomic_RNA.
DR   EMBL; AP006557; BAC81359.1; -; Genomic_RNA.
DR   EMBL; AP006558; BAC81373.1; -; Genomic_RNA.
DR   EMBL; AP006559; BAC81387.1; -; Genomic_RNA.
DR   EMBL; AP006560; BAC81401.1; -; Genomic_RNA.
DR   EMBL; AP006561; BAC81415.1; -; Genomic_RNA.
DR   EMBL; AY323977; AAP72985.1; -; Genomic_RNA.
DR   EMBL; AY427439; AAQ94071.1; -; Genomic_RNA.
DR   RefSeq; NP_828859.1; NC_004718.3.
DR   PDB; 2CME; X-ray; 2.80 A; A/B=9-98, E/G=9-98, C/D/F/H=10-98.
DR   PDBsum; 2CME; -.
DR   SMR; P59636; -.
DR   IntAct; P59636; 6.
DR   GeneID; 1489679; -.
DR   KEGG; vg:1489679; -.
DR   EvolutionaryTrace; P59636; -.
DR   Proteomes; UP000000354; Genome.
DR   Proteomes; UP000103670; Genome.
DR   Proteomes; UP000109640; Genome.
DR   Proteomes; UP000116947; Genome.
DR   Proteomes; UP000121636; Genome.
DR   Proteomes; UP000131569; Genome.
DR   Proteomes; UP000131955; Genome.
DR   Proteomes; UP000137377; Genome.
DR   Proteomes; UP000138690; Genome.
DR   Proteomes; UP000145651; Genome.
DR   Proteomes; UP000146108; Genome.
DR   Proteomes; UP000148194; Genome.
DR   Proteomes; UP000153467; Genome.
DR   Proteomes; UP000160648; Genome.
DR   Proteomes; UP000172416; Genome.
DR   GO; GO:0044162; C:host cell cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   InterPro; IPR018542; SARS_lipid-bd.
DR   InterPro; IPR037223; SARS_ORF-9b.
DR   Pfam; PF09399; SARS_lipid_bind; 1.
DR   SUPFAM; SSF141666; SSF141666; 1.
DR   PROSITE; PS51920; SARS_9B; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Host cytoplasm; Host cytoplasmic vesicle; Host membrane;
KW   Membrane; Reference proteome.
FT   CHAIN           1..98
FT                   /note="Protein 9b"
FT                   /id="PRO_0000106137"
FT   DOMAIN          9..98
FT                   /note="9b"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01268"
FT   VARIANT         58
FT                   /note="A -> V (in strain: Isolate TWK)"
FT   STRAND          14..23
FT                   /evidence="ECO:0000244|PDB:2CME"
FT   STRAND          42..47
FT                   /evidence="ECO:0000244|PDB:2CME"
FT   STRAND          54..60
FT                   /evidence="ECO:0000244|PDB:2CME"
FT   STRAND          65..67
FT                   /evidence="ECO:0000244|PDB:2CME"
FT   STRAND          69..74
FT                   /evidence="ECO:0000244|PDB:2CME"
FT   HELIX           85..87
FT                   /evidence="ECO:0000244|PDB:2CME"
FT   STRAND          90..97
FT                   /evidence="ECO:0000244|PDB:2CME"
SQ   SEQUENCE   98 AA;  10802 MW;  5166F22872915FCB CRC64;
     MDPNQTNVVP PALHLVDPQI QLTITRMEDA MGQGQNSADP KVYPIILRLG SQLSLSMARR
     NLDSLEARAF QSTPIVVQMT KLATTEELPD EFVVVTAK
//