ID   ORF9B_CVHSA             Reviewed;          98 AA.
AC   P59636; Q7T6R2;
DT   23-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT   23-APR-2003, sequence version 1.
DT   12-AUG-2020, entry version 93.
DE   RecName: Full=ORF9b protein;
DE   AltName: Full=Accessory protein 9b;
DE   AltName: Full=ORF-9b;
DE   AltName: Full=Protein 9b;
GN   ORFNames=9b;
OS   Human SARS coronavirus (SARS-CoV) (Severe acute respiratory syndrome
OS   coronavirus).
OC   Viruses; Riboviria; Nidovirales; Cornidovirineae; Coronaviridae;
OC   Orthocoronavirinae; Betacoronavirus; Sarbecovirus.
OX   NCBI_TaxID=694009;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
OH   NCBI_TaxID=9675; Paguma larvata (Masked palm civet).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate Urbani;
RX   PubMed=12730500; DOI=10.1126/science.1085952;
RA   Rota P.A., Oberste M.S., Monroe S.S., Nix W.A., Campagnoli R.,
RA   Icenogle J.P., Penaranda S., Bankamp B., Maher K., Chen M.-H., Tong S.,
RA   Tamin A., Lowe L., Frace M., DeRisi J.L., Chen Q., Wang D., Erdman D.D.,
RA   Peret T.C.T., Burns C., Ksiazek T.G., Rollin P.E., Sanchez A., Liffick S.,
RA   Holloway B., Limor J., McCaustland K., Olsen-Rasmussen M., Fouchier R.,
RA   Guenther S., Osterhaus A.D.M.E., Drosten C., Pallansch M.A., Anderson L.J.,
RA   Bellini W.J.;
RT   "Characterization of a novel coronavirus associated with severe acute
RT   respiratory syndrome.";
RL   Science 300:1394-1399(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate Tor2;
RX   PubMed=12730501; DOI=10.1126/science.1085953;
RA   Marra M.A., Jones S.J.M., Astell C.R., Holt R.A., Brooks-Wilson A.,
RA   Butterfield Y.S.N., Khattra J., Asano J.K., Barber S.A., Chan S.Y.,
RA   Cloutier A., Coughlin S.M., Freeman D., Girn N., Griffith O.L., Leach S.R.,
RA   Mayo M., McDonald H., Montgomery S.B., Pandoh P.K., Petrescu A.S.,
RA   Robertson A.G., Schein J.E., Siddiqui A., Smailus D.E., Stott J.M.,
RA   Yang G.S., Plummer F., Andonov A., Artsob H., Bastien N., Bernard K.,
RA   Booth T.F., Bowness D., Czub M., Drebot M., Fernando L., Flick R.,
RA   Garbutt M., Gray M., Grolla A., Jones S., Feldmann H., Meyers A.,
RA   Kabani A., Li Y., Normand S., Stroher U., Tipples G.A., Tyler S.,
RA   Vogrig R., Ward D., Watson B., Brunham R.C., Krajden M., Petric M.,
RA   Skowronski D.M., Upton C., Roper R.L.;
RT   "The genome sequence of the SARS-associated coronavirus.";
RL   Science 300:1399-1404(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate CUHK-Su10, and Isolate CUHK-W1;
RX   PubMed=12853594; DOI=10.1056/nejm200307103490216;
RA   Tsui S.K.W., Chim S.S.C., Lo Y.M.D.;
RT   "Coronavirus genomic-sequence variations and the epidemiology of the severe
RT   acute respiratory syndrome.";
RL   N. Engl. J. Med. 349:187-188(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate HKU-39849;
RX   PubMed=12876307; DOI=10.1177/15353702-0322807-13;
RA   Zeng F.Y., Chan C.W., Chan M.N., Chen J.D., Chow K.Y.C., Hon C.C.C.,
RA   Hui R.K.H., Li J., Li V.Y.Y., Wang C.Y., Wang P.Y., Guan Y., Zheng B.,
RA   Poon L.L.M., Chan K.H., Yuen K.Y., Peiris J.S.M., Leung F.C.;
RT   "The complete genome sequence of severe acute respiratory syndrome
RT   coronavirus strain HKU-39849 (HK-39).";
RL   Exp. Biol. Med. 228:866-873(2003).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate BJ01, Isolate BJ02, Isolate BJ03, Isolate BJ04, and
RC   Isolate GD01;
RA   Qin E., Zhu Q., Yu M., Fan B., Chang G., Si B., Yang B., Peng W., Jiang T.,
RA   Liu B., Deng Y., Liu H., Zhang Y., Wang C., Li Y., Gan Y., Li X., Lu F.,
RA   Tan G., Yang R., Cao W.S., Wang J., Chen W., Cong L., Deng Y., Dong W.,
RA   Han Y., Hu W., Lei M., Li C., Li G., Li G., Li H., Li S., Li S., Li W.,
RA   Li W., Lin W., Liu J., Liu Z., Lu H., Ni P., Qi Q., Sun Y., Tang L.,
RA   Tong Z., Wang J., Wang X., Wu Q., Xi Y., Xu Z., Yang L., Ye C., Ye J.,
RA   Zhang B., Zhang F., Zhang J., Zhang X., Zhou J., Yang H.;
RL   Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate TW1;
RA   Yeh S.-H., Kao C.-L., Tsai C.-Y., Liu C.-J., Chen D.-S., Chen P.-J.;
RT   "The complete genome of SARS coronavirus clone TW1.";
RL   Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN   [7]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate FRA;
RA   Eickmann M., Becker S., Klenk H.-D., Doerr H.W., Stadler K., Censini S.,
RA   Guidotti S., Masignani V., Scarselli M., Mora M., Donati C., Han J.,
RA   Song H.C., Abrignani S., Covacci A., Rappuoli R.;
RT   "SARS virus is a close relative of type II coronaviruses.";
RL   Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN   [8]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate Frankfurt 1;
RX   PubMed=12917450; DOI=10.1099/vir.0.19424-0;
RA   Thiel V., Ivanov K.A., Putics A., Hertzig T., Schelle B., Bayer S.,
RA   Weissbrich B., Snijder E.J., Rabenau H., Doerr H.W., Gorbalenya A.E.,
RA   Ziebuhr J.;
RT   "Mechanisms and enzymes involved in SARS coronavirus genome expression.";
RL   J. Gen. Virol. 84:2305-2315(2003).
RN   [9]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate ZJ01;
RX   PubMed=14527350;
RA   Li L., Wang Z., Lu Y., Bao Q., Chen S., Wu N., Cheng S., Weng J., Zhang Y.,
RA   Yan J., Mei L., Wang X., Zhu H., Yu Y., Zhang M., Li M., Yao J., Lu Q.,
RA   Yao P., Bo X., Wo J., Wang S., Hu S.;
RT   "Severe acute respiratory syndrome-associated coronavirus genotype and its
RT   characterization.";
RL   Chin. Med. J. 116:1288-1292(2003).
RN   [10]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate TWH, Isolate TWJ, Isolate TWK, Isolate TWS, and Isolate TWY;
RA   Shu H.Y., Wu K.M., Tsai S.F.;
RT   "The complete genome of SARS coronavirus TWH.";
RL   Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
RN   [11]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate HSR 1;
RA   Canducci F., Clementi M., Poli G., Vicenzi E.;
RL   Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
RN   [12]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate AS;
RA   Balotta C., Corvasce S., Violin M., Galli M., Moroni M., Vigevani G.M.,
RA   Ruan Y.J., Salemi M.;
RL   Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
RN   [13]
RP   SUBCELLULAR LOCATION, AND REGION.
RX   PubMed=17448558; DOI=10.1016/j.virusres.2007.03.011;
RA   Moshynskyy I., Viswanathan S., Vasilenko N., Lobanov V., Petric M.,
RA   Babiuk L.A., Zakhartchouk A.N.;
RT   "Intracellular localization of the SARS coronavirus protein 9b: evidence of
RT   active export from the nucleus.";
RL   Virus Res. 127:116-121(2007).
RN   [14]
RP   SUBCELLULAR LOCATION.
RX   PubMed=19394665; DOI=10.1016/j.virol.2009.03.032;
RA   Xu K., Zheng B.J., Zeng R., Lu W., Lin Y.P., Xue L., Li L., Yang L.L.,
RA   Xu C., Dai J., Wang F., Li Q., Dong Q.X., Yang R.F., Wu J.R., Sun B.;
RT   "Severe acute respiratory syndrome coronavirus accessory protein 9b is a
RT   virion-associated protein.";
RL   Virology 388:279-285(2009).
RN   [15]
RP   SUBCELLULAR LOCATION, AND INTERACTION WITH HOST XPO1.
RX   PubMed=21637748; DOI=10.1371/journal.pone.0019436;
RA   Sharma K., Aakerstroem S., Sharma A.K., Chow V.T., Teow S., Abrenica B.,
RA   Booth S.A., Booth T.F., Mirazimi A., Lal S.K.;
RT   "SARS-CoV 9b protein diffuses into nucleus, undergoes active Crm1 mediated
RT   nucleocytoplasmic export and triggers apoptosis when retained in the
RT   nucleus.";
RL   PLoS ONE 6:e19436-e19436(2011).
RN   [16]
RP   FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH HOST MAVS.
RX   PubMed=25135833; DOI=10.4049/jimmunol.1303196;
RA   Shi C.S., Qi H.Y., Boularan C., Huang N.N., Abu-Asab M., Shelhamer J.H.,
RA   Kehrl J.H.;
RT   "SARS-coronavirus open reading frame-9b suppresses innate immunity by
RT   targeting mitochondria and the MAVS/TRAF3/TRAF6 signalosome.";
RL   J. Immunol. 193:3080-3089(2014).
RN   [17]
RP   INTERACTION WITH PROTEIN ORF6, AND SUBCELLULAR LOCATION.
RX   PubMed=22820404; DOI=10.1016/j.virusres.2012.07.012;
RA   Calvo E., DeDiego M.L., Garcia P., Lopez J.A., Perez-Brena P., Falcon A.;
RT   "Severe acute respiratory syndrome coronavirus accessory proteins 6 and 9b
RT   interact in vivo.";
RL   Virus Res. 169:282-288(2012).
RN   [18]
RP   X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 9-98, AND SUBCELLULAR LOCATION.
RX   PubMed=16843897; DOI=10.1016/j.str.2006.05.012;
RA   Meier C., Aricescu A.R., Assenberg R., Aplin R.T., Gilbert R.J.C.,
RA   Grimes J.M., Stuart D.I.;
RT   "The crystal structure of ORF-9b, a lipid binding protein from the SARS
RT   coronavirus.";
RL   Structure 14:1157-1165(2006).
CC   -!- FUNCTION: Plays a role in the inhibition of host innate immune response
CC       by targeting the mitochondrial-associated adapter MAVS.
CC       Mechanistically, usurps the E3 ligase ITCH to trigger the degradation
CC       of MAVS, TRAF3, and TRAF6. In addition, causes mitochondrial elongation
CC       by triggering ubiquitination and proteasomal degradation of dynamin-
CC       like protein 1/DNM1L. {ECO:0000269|PubMed:25135833}.
CC   -!- SUBUNIT: Homodimer. Interacts with host XPO1; this interaction mediates
CC       protein ORF9b export out of the nucleus (PubMed:21637748). Interacts
CC       with host MAVS (PubMed:25135833). Interacts with protein ORF6
CC       (PubMed:22820404). {ECO:0000269|PubMed:21637748,
CC       ECO:0000269|PubMed:22820404, ECO:0000269|PubMed:25135833}.
CC   -!- INTERACTION:
CC       P59636; P59636: 9b; NbExp=3; IntAct=EBI-9021274, EBI-9021274;
CC       P59636; Q7TLC7: ORF14; NbExp=3; IntAct=EBI-9021274, EBI-25488942;
CC       P59636; PRO_0000037320 [P0C6X7]: rep; NbExp=3; IntAct=EBI-9021274, EBI-25487328;
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:19394665}. Host
CC       cytoplasmic vesicle membrane {ECO:0000269|PubMed:16843897}; Peripheral
CC       membrane protein {ECO:0000269|PubMed:16843897}. Host cytoplasm
CC       {ECO:0000269|PubMed:16843897, ECO:0000269|PubMed:21637748,
CC       ECO:0000269|PubMed:22820404}. Host endoplasmic reticulum
CC       {ECO:0000269|PubMed:17448558}. Host nucleus
CC       {ECO:0000269|PubMed:21637748}. Host mitochondrion
CC       {ECO:0000269|PubMed:25135833}. Note=Binds non-covalently to
CC       intracellular lipid bilayers.
CC   -!- MISCELLANEOUS: The gene encoding this protein is included within the N
CC       gene (alternative ORF).
CC   -!- SIMILARITY: Belongs to the coronavirus group 2 protein 9b family.
CC       {ECO:0000305}.
DR   EMBL; AY278741; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   EMBL; AY274119; AAP41048.1; -; Genomic_RNA.
DR   EMBL; AY282752; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   EMBL; AY278554; AAP13574.1; -; Genomic_RNA.
DR   EMBL; AY278491; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   EMBL; AY278487; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   EMBL; AY278488; AAP30038.1; -; Genomic_RNA.
DR   EMBL; AY278489; AAP51235.1; -; Genomic_RNA.
DR   EMBL; AY278490; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   EMBL; AY279354; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   EMBL; AY291451; AAP37025.1; -; Genomic_RNA.
DR   EMBL; AY310120; AAP50496.1; -; Genomic_RNA.
DR   EMBL; AY291315; AAP33708.1; -; Genomic_RNA.
DR   EMBL; AY290752; AAP69659.1; -; Genomic_RNA.
DR   EMBL; AP006557; BAC81359.1; -; Genomic_RNA.
DR   EMBL; AP006558; BAC81373.1; -; Genomic_RNA.
DR   EMBL; AP006559; BAC81387.1; -; Genomic_RNA.
DR   EMBL; AP006560; BAC81401.1; -; Genomic_RNA.
DR   EMBL; AP006561; BAC81415.1; -; Genomic_RNA.
DR   EMBL; AY323977; AAP72985.1; -; Genomic_RNA.
DR   EMBL; AY427439; AAQ94071.1; -; Genomic_RNA.
DR   RefSeq; NP_828859.1; NC_004718.3.
DR   PDB; 2CME; X-ray; 2.80 A; A/B=9-98, E/G=9-98, C/D/F/H=10-98.
DR   PDBsum; 2CME; -.
DR   SMR; P59636; -.
DR   BioGRID; 4383924; 19.
DR   IntAct; P59636; 6.
DR   GeneID; 1489679; -.
DR   KEGG; vg:1489679; -.
DR   SIGNOR; P59636; -.
DR   EvolutionaryTrace; P59636; -.
DR   Proteomes; UP000000354; Genome.
DR   Proteomes; UP000103670; Genome.
DR   Proteomes; UP000109640; Genome.
DR   Proteomes; UP000116947; Genome.
DR   Proteomes; UP000121636; Genome.
DR   Proteomes; UP000131569; Genome.
DR   Proteomes; UP000131955; Genome.
DR   Proteomes; UP000137377; Genome.
DR   Proteomes; UP000138690; Genome.
DR   Proteomes; UP000145651; Genome.
DR   Proteomes; UP000146108; Genome.
DR   Proteomes; UP000148194; Genome.
DR   Proteomes; UP000153467; Genome.
DR   Proteomes; UP000160648; Genome.
DR   Proteomes; UP000172416; Genome.
DR   GO; GO:0044162; C:host cell cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR   InterPro; IPR037223; Protein_9b_SARS.
DR   InterPro; IPR018542; Protein_9b_SARS-like.
DR   Pfam; PF09399; SARS_lipid_bind; 1.
DR   SUPFAM; SSF141666; SSF141666; 1.
DR   PROSITE; PS51920; SARS_9B; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Host cytoplasm; Host cytoplasmic vesicle;
KW   Host endoplasmic reticulum; Host membrane; Host mitochondrion;
KW   Host nucleus; Host-virus interaction;
KW   Inhibition of host innate immune response by virus;
KW   Inhibition of host MAVS by virus; Inhibition of host RLR pathway by virus;
KW   Membrane; Reference proteome; Viral immunoevasion; Virion.
FT   CHAIN           1..98
FT                   /note="ORF9b protein"
FT                   /id="PRO_0000106137"
FT   DOMAIN          9..98
FT                   /note="9b"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01268"
FT   MOTIF           46..54
FT                   /note="Nuclear export signal"
FT                   /evidence="ECO:0000269|PubMed:17448558"
FT   VARIANT         58
FT                   /note="A -> V (in strain: Isolate TWK)"
FT   STRAND          14..23
FT                   /evidence="ECO:0000244|PDB:2CME"
FT   STRAND          42..47
FT                   /evidence="ECO:0000244|PDB:2CME"
FT   STRAND          54..60
FT                   /evidence="ECO:0000244|PDB:2CME"
FT   STRAND          65..67
FT                   /evidence="ECO:0000244|PDB:2CME"
FT   STRAND          69..74
FT                   /evidence="ECO:0000244|PDB:2CME"
FT   HELIX           85..87
FT                   /evidence="ECO:0000244|PDB:2CME"
FT   STRAND          90..97
FT                   /evidence="ECO:0000244|PDB:2CME"
SQ   SEQUENCE   98 AA;  10802 MW;  5166F22872915FCB CRC64;
     MDPNQTNVVP PALHLVDPQI QLTITRMEDA MGQGQNSADP KVYPIILRLG SQLSLSMARR
     NLDSLEARAF QSTPIVVQMT KLATTEELPD EFVVVTAK
//