ID   VEMP_CVHSA              Reviewed;          76 AA.
AC   P59637; Q7TA13; Q7TFA8;
DT   23-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT   23-APR-2003, sequence version 1.
DT   12-AUG-2020, entry version 95.
DE   RecName: Full=Envelope small membrane protein {ECO:0000255|HAMAP-Rule:MF_04204};
DE            Short=E protein {ECO:0000255|HAMAP-Rule:MF_04204};
DE            Short=sM protein {ECO:0000255|HAMAP-Rule:MF_04204};
GN   Name=E {ECO:0000255|HAMAP-Rule:MF_04204}; Synonyms=sM; ORFNames=4;
OS   Human SARS coronavirus (SARS-CoV) (Severe acute respiratory syndrome
OS   coronavirus).
OC   Viruses; Riboviria; Nidovirales; Cornidovirineae; Coronaviridae;
OC   Orthocoronavirinae; Betacoronavirus; Sarbecovirus.
OX   NCBI_TaxID=694009;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
OH   NCBI_TaxID=9675; Paguma larvata (Masked palm civet).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate Urbani;
RX   PubMed=12730500; DOI=10.1126/science.1085952;
RA   Rota P.A., Oberste M.S., Monroe S.S., Nix W.A., Campagnoli R.,
RA   Icenogle J.P., Penaranda S., Bankamp B., Maher K., Chen M.-H., Tong S.,
RA   Tamin A., Lowe L., Frace M., DeRisi J.L., Chen Q., Wang D., Erdman D.D.,
RA   Peret T.C.T., Burns C., Ksiazek T.G., Rollin P.E., Sanchez A., Liffick S.,
RA   Holloway B., Limor J., McCaustland K., Olsen-Rasmussen M., Fouchier R.,
RA   Guenther S., Osterhaus A.D.M.E., Drosten C., Pallansch M.A., Anderson L.J.,
RA   Bellini W.J.;
RT   "Characterization of a novel coronavirus associated with severe acute
RT   respiratory syndrome.";
RL   Science 300:1394-1399(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate Tor2;
RX   PubMed=12730501; DOI=10.1126/science.1085953;
RA   Marra M.A., Jones S.J.M., Astell C.R., Holt R.A., Brooks-Wilson A.,
RA   Butterfield Y.S.N., Khattra J., Asano J.K., Barber S.A., Chan S.Y.,
RA   Cloutier A., Coughlin S.M., Freeman D., Girn N., Griffith O.L., Leach S.R.,
RA   Mayo M., McDonald H., Montgomery S.B., Pandoh P.K., Petrescu A.S.,
RA   Robertson A.G., Schein J.E., Siddiqui A., Smailus D.E., Stott J.M.,
RA   Yang G.S., Plummer F., Andonov A., Artsob H., Bastien N., Bernard K.,
RA   Booth T.F., Bowness D., Czub M., Drebot M., Fernando L., Flick R.,
RA   Garbutt M., Gray M., Grolla A., Jones S., Feldmann H., Meyers A.,
RA   Kabani A., Li Y., Normand S., Stroher U., Tipples G.A., Tyler S.,
RA   Vogrig R., Ward D., Watson B., Brunham R.C., Krajden M., Petric M.,
RA   Skowronski D.M., Upton C., Roper R.L.;
RT   "The genome sequence of the SARS-associated coronavirus.";
RL   Science 300:1399-1404(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate CUHK-Su10, and Isolate CUHK-W1;
RX   PubMed=12853594; DOI=10.1056/nejm200307103490216;
RA   Tsui S.K.W., Chim S.S.C., Lo Y.M.D.;
RT   "Coronavirus genomic-sequence variations and the epidemiology of the severe
RT   acute respiratory syndrome.";
RL   N. Engl. J. Med. 349:187-188(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate GZ50, and Isolate HKU-36871;
RX   PubMed=12958366; DOI=10.1126/science.1087139;
RA   Guan Y., Zheng B.J., He Y.Q., Liu X.L., Zhuang Z.X., Cheung C.L., Luo S.W.,
RA   Li P.H., Zhang L.J., Guan Y.J., Butt K.M., Wong K.L., Chan K.W., Lim W.,
RA   Shortridge K.F., Yuen K.Y., Peiris J.S.M., Poon L.L.M.;
RT   "Isolation and characterization of viruses related to the SARS coronavirus
RT   from animals in southern China.";
RL   Science 302:276-278(2003).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate HKU-39849;
RX   PubMed=12876307; DOI=10.1177/15353702-0322807-13;
RA   Zeng F.Y., Chan C.W., Chan M.N., Chen J.D., Chow K.Y.C., Hon C.C.C.,
RA   Hui R.K.H., Li J., Li V.Y.Y., Wang C.Y., Wang P.Y., Guan Y., Zheng B.,
RA   Poon L.L.M., Chan K.H., Yuen K.Y., Peiris J.S.M., Leung F.C.;
RT   "The complete genome sequence of severe acute respiratory syndrome
RT   coronavirus strain HKU-39849 (HK-39).";
RL   Exp. Biol. Med. 228:866-873(2003).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate Sin2500, Isolate Sin2677, Isolate Sin2679, Isolate Sin2748,
RC   and Isolate sin2774;
RX   PubMed=12781537; DOI=10.1016/s0140-6736(03)13414-9;
RA   Ruan Y., Wei C.L., Ling A.E., Vega V.B., Thoreau H., Se Thoe S.Y.,
RA   Chia J.-M., Ng P., Chiu K.P., Lim L., Zhang T., Chan K.P., Oon L.E.L.,
RA   Ng M.L., Leo S.Y., Ng L.F.P., Ren E.C., Stanton L.W., Long P.M., Liu E.T.;
RT   "Comparative full-length genome sequence analysis of 14 SARS coronavirus
RT   isolates and common mutations associated with putative origins of
RT   infection.";
RL   Lancet 361:1779-1785(2003).
RN   [7]
RP   ERRATUM OF PUBMED:12781537.
RA   Ruan Y., Wei C.L., Ling A.E., Vega V.B., Thoreau H., Se Thoe S.Y.,
RA   Chia J.-M., Ng P., Chiu K.P., Lim L., Zhang T., Chan K.P., Oon L.E.L.,
RA   Ng M.L., Leo S.Y., Ng L.F.P., Ren E.C., Stanton L.W., Long P.M., Liu E.T.;
RL   Lancet 361:1832-1832(2003).
RN   [8]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate BJ01, Isolate BJ02, Isolate BJ03, Isolate BJ04, and
RC   Isolate GD01;
RA   Qin E., Zhu Q., Yu M., Fan B., Chang G., Si B., Yang B., Peng W., Jiang T.,
RA   Liu B., Deng Y., Liu H., Zhang Y., Wang C., Li Y., Gan Y., Li X., Lu F.,
RA   Tan G., Yang R., Cao W.S., Wang J., Chen W., Cong L., Deng Y., Dong W.,
RA   Han Y., Hu W., Lei M., Li C., Li G., Li G., Li H., Li S., Li S., Li W.,
RA   Li W., Lin W., Liu J., Liu Z., Lu H., Ni P., Qi Q., Sun Y., Tang L.,
RA   Tong Z., Wang J., Wang X., Wu Q., Xi Y., Xu Z., Yang L., Ye C., Ye J.,
RA   Zhang B., Zhang F., Zhang J., Zhang X., Zhou J., Yang H.;
RL   Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases.
RN   [9]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate TW1;
RA   Yeh S.-H., Kao C.-L., Tsai C.-Y., Liu C.-J., Chen D.-S., Chen P.-J.;
RT   "The complete genome of SARS coronavirus clone TW1.";
RL   Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN   [10]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate FRA;
RA   Eickmann M., Becker S., Klenk H.-D., Doerr H.W., Stadler K., Censini S.,
RA   Guidotti S., Masignani V., Scarselli M., Mora M., Donati C., Han J.,
RA   Song H.C., Abrignani S., Covacci A., Rappuoli R.;
RT   "SARS virus is a close relative of type II coronaviruses.";
RL   Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN   [11]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate Frankfurt 1;
RX   PubMed=12917450; DOI=10.1099/vir.0.19424-0;
RA   Thiel V., Ivanov K.A., Putics A., Hertzig T., Schelle B., Bayer S.,
RA   Weissbrich B., Snijder E.J., Rabenau H., Doerr H.W., Gorbalenya A.E.,
RA   Ziebuhr J.;
RT   "Mechanisms and enzymes involved in SARS coronavirus genome expression.";
RL   J. Gen. Virol. 84:2305-2315(2003).
RN   [12]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate ZJ01;
RX   PubMed=14527350;
RA   Li L., Wang Z., Lu Y., Bao Q., Chen S., Wu N., Cheng S., Weng J., Zhang Y.,
RA   Yan J., Mei L., Wang X., Zhu H., Yu Y., Zhang M., Li M., Yao J., Lu Q.,
RA   Yao P., Bo X., Wo J., Wang S., Hu S.;
RT   "Severe acute respiratory syndrome-associated coronavirus genotype and its
RT   characterization.";
RL   Chin. Med. J. 116:1288-1292(2003).
RN   [13]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Isolate ZJ01;
RA   Cong L.-M., Ding G.-Q., Lu Y.-Y., Weng J.-Q., Yan J.-Y., Hu N.-P.,
RA   Wo J.-E., Chen S.-Y., Zhang Y.-J., Mei L.-L., Wang Z.-G., Yao J.,
RA   Zhu H.-P., Lu Q.-Y., Li M.-H., Gong L.-M., Shi W., Li L.-J.;
RT   "SARS coronavirus ZJ01 isolate small envelope E protein.";
RL   Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases.
RN   [14]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate TWC;
RA   Yang J.-Y., Lin J.-H., Chiu S.-C., Wang S.-F., Lee S.C., Lin Y.-C.,
RA   Hsu C.-K., Chen H.-Y., Chang J.G., Chen P.-J., Su I.-J.;
RT   "Genomic sequence of SARS isolate from the first fatal case in Taiwan.";
RL   Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases.
RN   [15]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate Shanghai QXC1;
RA   Yuan Z., Zhang X., Hu Y., Lan S., Wang H., Zhou Z., Wen Y.;
RT   "Analysis of SARS coronavirus genome in Shanghai isolates.";
RL   Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN   [16]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate Taiwan TC1, Isolate Taiwan TC2, and Isolate Taiwan TC3;
RA   Chang J.-G.C., Lin T.-H., Chen C.-M., Lin C.-S., Chan W.-L., Shih M.-C.;
RL   Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
RN   [17]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate TWH, Isolate TWJ, Isolate TWK, Isolate TWS, and Isolate TWY;
RA   Shu H.Y., Wu K.M., Tsai S.F.;
RT   "The complete genome of SARS coronavirus TWH.";
RL   Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
RN   [18]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate HSR 1;
RA   Canducci F., Clementi M., Poli G., Vicenzi E.;
RL   Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
RN   [19]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate TWC2, and Isolate TWC3;
RA   Yang J.-Y., Lin J.-H., Chiu S.-C., Wang S.-F., Lee H.-C., Lin Y.-C.,
RA   Hsu C.-K., Chen H.-Y., Chen P.-J., Su I.-J.;
RL   Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
RN   [20]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate AS;
RA   Balotta C., Corvasce S., Violin M., Galli M., Moroni M., Vigevani G.M.,
RA   Ruan Y.J., Salemi M.;
RL   Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
RN   [21]
RP   INTERACTION WITH ACCESSORY PROTEIN 3A.
RX   PubMed=15194747; DOI=10.1128/jvi.78.13.6723-6734.2004;
RA   Tan Y.-J., Teng E., Shen S., Tan T.H.P., Goh P.-Y., Fielding B.C.,
RA   Ooi E.-E., Tan H.-C., Lim S.G., Hong W.;
RT   "A novel severe acute respiratory syndrome coronavirus protein, U274, is
RT   transported to the cell surface and undergoes endocytosis.";
RL   J. Virol. 78:6723-6734(2004).
RN   [22]
RP   INTERACTION WITH ACCESSORY PROTEIN 3A.
RX   PubMed=15763150; DOI=10.1016/j.virusres.2005.01.001;
RA   Yuan X., Li J., Shan Y., Yang Z., Zhao Z., Chen B., Yao Z., Dong B.,
RA   Wang S., Chen J., Cong Y.;
RT   "Subcellular localization and membrane association of SARS-CoV 3a
RT   protein.";
RL   Virus Res. 109:191-202(2005).
RN   [23]
RP   INTERACTION WITH ACCESSORY PROTEIN 7A.
RX   PubMed=16580632; DOI=10.1016/j.bbrc.2006.03.091;
RA   Fielding B.C., Gunalan V., Tan T.H.P., Chou C.-F., Shen S., Khan S.,
RA   Lim S.G., Hong W., Tan Y.-J.;
RT   "Severe acute respiratory syndrome coronavirus protein 7a interacts with
RT   hSGT.";
RL   Biochem. Biophys. Res. Commun. 343:1201-1208(2006).
RN   [24]
RP   INTERACTION WITH PROTEIN M.
RX   PubMed=19322648; DOI=10.1007/s11262-009-0341-6;
RA   Chen S.C., Lo S.Y., Ma H.C., Li H.C.;
RT   "Expression and membrane integration of SARS-CoV E protein and its
RT   interaction with M protein.";
RL   Virus Genes 38:365-371(2009).
RN   [25]
RP   INTERACTION WITH HUMAN MPP5, SUBCELLULAR LOCATION, MOTIF, AND MUTAGENESIS
RP   OF 73-ASP--VAL-76.
RX   PubMed=20861307; DOI=10.1091/mbc.e10-04-0338;
RA   Teoh K.T., Siu Y.L., Chan W.L., Schlueter M.A., Liu C.J., Peiris J.S.,
RA   Bruzzone R., Margolis B., Nal B.;
RT   "The SARS coronavirus E protein interacts with PALS1 and alters tight
RT   junction formation and epithelial morphogenesis.";
RL   Mol. Biol. Cell 21:3838-3852(2010).
RN   [26]
RP   SUBCELLULAR LOCATION.
RX   PubMed=21450821; DOI=10.1128/jvi.00060-11;
RA   Cohen J.R., Lin L.D., Machamer C.E.;
RT   "Identification of a Golgi complex-targeting signal in the cytoplasmic tail
RT   of the severe acute respiratory syndrome coronavirus envelope protein.";
RL   J. Virol. 85:5794-5803(2011).
RN   [27]
RP   SUBUNIT.
RX   PubMed=22819936; DOI=10.1016/j.pep.2012.07.005;
RA   Parthasarathy K., Lu H., Surya W., Vararattanavech A., Pervushin K.,
RA   Torres J.;
RT   "Expression and purification of coronavirus envelope proteins using a
RT   modified beta-barrel construct.";
RL   Protein Expr. Purif. 85:133-141(2012).
RN   [28]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=24788150; DOI=10.1371/journal.ppat.1004077;
RA   Nieto-Torres J.L., DeDiego M.L., Verdia-Baguena C., Jimenez-Guardeno J.M.,
RA   Regla-Nava J.A., Fernandez-Delgado R., Castano-Rodriguez C., Alcaraz A.,
RA   Torres J., Aguilella V.M., Enjuanes L.;
RT   "Severe acute respiratory syndrome coronavirus envelope protein ion channel
RT   activity promotes virus fitness and pathogenesis.";
RL   PLoS Pathog. 10:E1004077-E1004077(2014).
RN   [29]
RP   INTERACTION WITH NUCLEOPROTEIN.
RX   PubMed=24766657; DOI=10.1186/1423-0127-21-34;
RA   Tseng Y.T., Wang S.M., Huang K.J., Wang C.T.;
RT   "SARS-CoV envelope protein palmitoylation or nucleocapid association is not
RT   required for promoting virus-like particle production.";
RL   J. Biomed. Sci. 21:34-34(2014).
RN   [30]
RP   FUNCTION.
RX   PubMed=26331680; DOI=10.1016/j.virol.2015.08.010;
RA   Nieto-Torres J.L., Verdia-Baguena C., Jimenez-Guardeno J.M.,
RA   Regla-Nava J.A., Castano-Rodriguez C., Fernandez-Delgado R., Torres J.,
RA   Aguilella V.M., Enjuanes L.;
RT   "Severe acute respiratory syndrome coronavirus E protein transports calcium
RT   ions and activates the NLRP3 inflammasome.";
RL   Virology 485:330-339(2015).
CC   -!- FUNCTION: Plays a central role in virus morphogenesis and assembly.
CC       Acts as a viroporin and self-assembles in host membranes forming
CC       pentameric protein-lipid pores that allow ion transport. Also plays a
CC       role in the induction of apoptosis (By similarity). Activates the host
CC       NLRP3 inflammasome, leading to IL-1beta overproduction.
CC       {ECO:0000255|HAMAP-Rule:MF_04204, ECO:0000269|PubMed:24788150,
CC       ECO:0000269|PubMed:26331680}.
CC   -!- SUBUNIT: Homopentamer (PubMed:22819936). Interacts with membrane
CC       protein M in the budding compartment of the host cell, which is located
CC       between endoplasmic reticulum and the Golgi complex (PubMed:19322648).
CC       Interacts with Nucleoprotein (PubMed:24766657). Interacts with the
CC       accessory proteins 3a and 7a. Interacts (via C-terminus) with human
CC       MPP5 (via PDZ domain); this inhibits the interaction between human MPP5
CC       and human CRB3, and causes delayed tight junction formation and
CC       defective cell polarity (PubMed:20861307). {ECO:0000255|HAMAP-
CC       Rule:MF_04204, ECO:0000269|PubMed:15194747,
CC       ECO:0000269|PubMed:15763150, ECO:0000269|PubMed:16580632,
CC       ECO:0000269|PubMed:20861307, ECO:0000269|PubMed:22819936}.
CC   -!- INTERACTION:
CC       P59637; Q7TFA1: 7b; NbExp=2; IntAct=EBI-25487741, EBI-25492846;
CC       P59637; P59637: E; NbExp=2; IntAct=EBI-25487741, EBI-25487741;
CC       P59637; PRO_0000037309 [P0C6X7]: rep; NbExp=2; IntAct=EBI-25487741, EBI-25475797;
CC       P59637; PRO_0000037315 [P0C6X7]: rep; NbExp=2; IntAct=EBI-25487741, EBI-25487941;
CC       P59637; O35843: Bcl2l1; Xeno; NbExp=2; IntAct=EBI-25487741, EBI-25487771;
CC       P59637; Q64373: Bcl2l1; Xeno; NbExp=2; IntAct=EBI-25487741, EBI-526361;
CC   -!- SUBCELLULAR LOCATION: Host endoplasmic reticulum-Golgi intermediate
CC       compartment {ECO:0000269|PubMed:20861307}. Host Golgi apparatus
CC       membrane {ECO:0000255|HAMAP-Rule:MF_04204, ECO:0000269|PubMed:21450821,
CC       ECO:0000269|PubMed:24788150}; Single-pass type III membrane protein
CC       {ECO:0000255|HAMAP-Rule:MF_04204}. Note=Colocalizes with S in the host
CC       endoplasmic reticulum-Golgi intermediate compartment (PubMed:20861307).
CC       The cytoplasmic tail functions as a Golgi complex-targeting signal.
CC       {ECO:0000255|HAMAP-Rule:MF_04204, ECO:0000269|PubMed:20861307,
CC       ECO:0000269|PubMed:21450821}.
CC   -!- SIMILARITY: Belongs to the betacoronaviruses E protein family.
CC       {ECO:0000255|HAMAP-Rule:MF_04204}.
DR   EMBL; AY278741; AAP13443.1; -; Genomic_RNA.
DR   EMBL; AY274119; AAP41040.1; -; Genomic_RNA.
DR   EMBL; AY304492; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   EMBL; AY282752; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   EMBL; AY278554; AAP13570.1; -; Genomic_RNA.
DR   EMBL; AY278491; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   EMBL; AY283794; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   EMBL; AY283795; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   EMBL; AY283796; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   EMBL; AY283797; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   EMBL; AY283798; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   EMBL; AY278487; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   EMBL; AY278488; AAP30033.1; -; Genomic_RNA.
DR   EMBL; AY278489; AAP51230.1; -; Genomic_RNA.
DR   EMBL; AY278490; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   EMBL; AY279354; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   EMBL; AY291451; AAP37020.1; -; Genomic_RNA.
DR   EMBL; AY310120; AAP50488.1; -; Genomic_RNA.
DR   EMBL; AY291315; AAP33700.1; -; Genomic_RNA.
DR   EMBL; AY297028; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   EMBL; AY323975; AAP73416.1; -; mRNA.
DR   EMBL; AY321118; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   EMBL; AY463059; AAP82979.2; -; Genomic_RNA.
DR   EMBL; AY338174; AAQ01600.1; -; Genomic_RNA.
DR   EMBL; AY338175; AAQ01612.1; -; Genomic_RNA.
DR   EMBL; AY348314; AAP97885.1; -; Genomic_RNA.
DR   EMBL; AP006557; BAC81351.1; -; Genomic_RNA.
DR   EMBL; AP006558; BAC81365.1; -; Genomic_RNA.
DR   EMBL; AP006559; BAC81379.1; -; Genomic_RNA.
DR   EMBL; AP006560; BAC81393.1; -; Genomic_RNA.
DR   EMBL; AP006561; BAC81407.1; -; Genomic_RNA.
DR   EMBL; AY323977; AAP72977.1; -; Genomic_RNA.
DR   EMBL; AY362698; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   EMBL; AY362699; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   EMBL; AY427439; AAQ94063.1; -; Genomic_RNA.
DR   RefSeq; NP_828854.1; NC_004718.3.
DR   PDB; 2MM4; NMR; -; A=8-65.
DR   PDB; 5X29; NMR; -; A/B/C/D/E=8-65.
DR   PDB; 5XER; NMR; -; A=55-63.
DR   PDB; 5XES; NMR; -; A=55-63.
DR   PDBsum; 2MM4; -.
DR   PDBsum; 5X29; -.
DR   PDBsum; 5XER; -.
DR   PDBsum; 5XES; -.
DR   SMR; P59637; -.
DR   BioGRID; 4383914; 10.
DR   IntAct; P59637; 10.
DR   PRIDE; P59637; -.
DR   GeneID; 1489671; -.
DR   KEGG; vg:1489671; -.
DR   KO; K24151; -.
DR   SIGNOR; P59637; -.
DR   Proteomes; UP000000354; Genome.
DR   Proteomes; UP000103670; Genome.
DR   Proteomes; UP000109640; Genome.
DR   Proteomes; UP000116947; Genome.
DR   Proteomes; UP000121636; Genome.
DR   Proteomes; UP000131569; Genome.
DR   Proteomes; UP000131955; Genome.
DR   Proteomes; UP000137377; Genome.
DR   Proteomes; UP000138690; Genome.
DR   Proteomes; UP000143093; Genome.
DR   Proteomes; UP000145651; Genome.
DR   Proteomes; UP000146108; Genome.
DR   Proteomes; UP000146181; Genome.
DR   Proteomes; UP000146296; Genome.
DR   Proteomes; UP000148194; Genome.
DR   Proteomes; UP000153467; Genome.
DR   Proteomes; UP000160648; Genome.
DR   Proteomes; UP000164441; Genome.
DR   Proteomes; UP000172416; Genome.
DR   GO; GO:0044172; C:host cell endoplasmic reticulum-Golgi intermediate compartment; IEA:UniProtKB-SubCell.
DR   GO; GO:0044177; C:host cell Golgi apparatus; IDA:UniProtKB.
DR   GO; GO:0044178; C:host cell Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR   GO; GO:0044662; P:disruption by virus of host cell membrane; IDA:CACAO.
DR   GO; GO:0039707; P:pore formation by virus in membrane of host cell; IEA:InterPro.
DR   GO; GO:0046760; P:viral budding from Golgi membrane; IDA:UniProtKB.
DR   HAMAP; MF_04204; BETA_CORONA_E; 1.
DR   InterPro; IPR043506; E_protein_bCoV.
DR   InterPro; IPR003873; E_protein_CoV.
DR   Pfam; PF02723; NS3_envE; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Apoptosis; Host Golgi apparatus; Host membrane; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..76
FT                   /note="Envelope small membrane protein"
FT                   /id="PRO_0000106074"
FT   TOPO_DOM        1..16
FT                   /note="Virion surface"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04204"
FT   TRANSMEM        17..37
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04204"
FT   TOPO_DOM        38..76
FT                   /note="Intravirion"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04204"
FT   MOTIF           73..76
FT                   /note="PDZ-binding; required for interaction with human
FT                   MPP5"
FT                   /evidence="ECO:0000269|PubMed:20861307"
FT   VARIANT         24
FT                   /note="V -> M (in strain: Isolate GD01)"
FT   MUTAGEN         73..76
FT                   /note="Missing: Abolishes interaction with PDZ domain of
FT                   human MPP5; loss of accumulation in host perinuclear
FT                   compartment."
FT                   /evidence="ECO:0000269|PubMed:20861307"
FT   HELIX           14..42
FT                   /evidence="ECO:0000244|PDB:2MM4"
FT   TURN            43..45
FT                   /evidence="ECO:0000244|PDB:2MM4"
FT   HELIX           56..64
FT                   /evidence="ECO:0000244|PDB:2MM4"
SQ   SEQUENCE   76 AA;  8361 MW;  A6D9A31652DDA194 CRC64;
     MYSFVSEETG TLIVNSVLLF LAFVVFLLVT LAILTALRLC AYCCNIVNVS LVKPTVYVYS
     RVKNLNSSEG VPDLLV
//